For reasons not clearly understood, Acute Lung Injury (ALI), often caused by systemic infections or severe trauma, leads to Acute Respiratory Distress Syndrome (ARDS), which is characterized by pulmonary edema and leukocyte infiltration, and affects 100,000 Americans annually. Phospholipase A2 (PLA2) enzyme, able to cleave phospholipids, is the target of signal transduction cascades, and plays a role in several bodily functions. PLA2 group 2A specifically hydrolyzes surfactant phospholipids invitro. The objective of this study is to determine whether PLA2 enzymes are produced and subsequently secreted by A549 lung epithelial cells in response to cytokine stimulation (and, more generally, to infection or ALI). It was found that TNFα + IL-6 stimulation in particular induces increased production but not necessarily secretion of PLA2 in A549 cells. This finding supports the hypothesis that PLA2 enzymes help mediate the inflammatory responses initiated in ARDS.
"Production of Phospholipase A2 in A549 Human Lung Epithelial Cells is Induced by Cytokines,"
Colgate Academic Review:
Vol. 8, Article 12.
Available at: http://commons.colgate.edu/car/vol8/iss1/12